Journal: International Journal of Surgery (London, England)
Article Title: Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway
doi: 10.1097/JS9.0000000000002075
Figure Lengend Snippet: POL stimulated BMSCs proliferation and osteogenic differentiation. (A) POL chemical structure; (B) BMSCs surface markers were detected by flow cytometry; (C) The result of CCK-8 assay ( n =5); (D, E) The result of CFU assay ( n =5); (F) Images of ALP staining (scale bar=250 μm); (G) Quantification of ALP staining ( n =5); (H) Images of ARS staining (scale bar=250 μm); (I) Quantification of ARS staining ( n =5); (J, K) The protein expression levels of OCN, RUNX2, ALP, and COL1A1 were evaluated by western blot ( n =3); (L, M) Immunofluorescence staining of OCN and ALP (scale bar=200 μm). Data were presented as mean±SEM. Compared with control group: * P <0.05, ** P <0.01, *** P <0.001. Compared with 1 μM group: # P <0.05, ## P <0.01, ### P <0.001.
Article Snippet: Primary antibodies against osteocalcin (OCN), runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), collagen type I alpha 1 (COL1A1), vascular endothelial growth factor A (VEGFA), platelet endothelial cell adhesion molecule-1 (CD31), endomucin (EMCN), PI3K, phosphorylated-PI3K (p-PI3K), AKT, phosphorylated-AKT (p-AKT), GSK-3β and phosphorylated-GSK-3β (p-GSK-3β), and GAPDH were from Affinity Biosciences (Cincinnati, USA).
Techniques: Flow Cytometry, CCK-8 Assay, Colony-forming Unit Assay, Staining, Expressing, Western Blot, Immunofluorescence, Control